Cellular & Longevity
NAD+
Nicotinamide Adenine Dinucleotide
Cellular energy and repair support
Quick answer
A 500 mg vial with 5 mL of water makes 100 mg/mL. A 50 mg dose is 50 units. Push it slowly. Two or three times a week.
- Mix it
- 500 mg vial + 5 mL water
- Draw
- About 50 units on a U-100 syringe
- How often
- 2 to 3 times a week
- How it is given
- Under the skin, injected very slowly.
- How long it lasts
- Short, but the effect on cell energy lasts longer.
- Store it
- Refrigerate. Keep out of light. Use within about 4 weeks.
What it is
Full name: Nicotinamide Adenine Dinucleotide. Most people write it as NAD+.
NAD+ is a molecule every cell uses to make energy and repair DNA. Levels drop with age. Vials are very large compared to peptides, usually 500 mg or 1000 mg.
What it is studied for
- Energy and mental clarity
- Cellular repair
- Age-related decline
- Recovery from heavy training
These are things researchers study. They are not promised results.
Dosing at a glance
These are the amounts used most often in research. Always start at the low end.
- Vial size
- 500 mg or 1000 mg
- Dose range
- 50 mg to 100 mg per dose
- How often
- 2 to 3 times a week
- Cycle length
- Ongoing, with breaks
- Mix with
- Bacteriostatic water (also called BAC water or reconstitution solution).
- Storage
- Refrigerate. Keep out of light. Use within about 4 weeks.
Week by week
A common way researchers step the dose up. Stay at each step until it feels easy.
| When | Dose | Notes |
|---|---|---|
| Week 1 | 25 mg, 2 times a week | Half dose to test tolerance. |
| Weeks 2-4 | 50 mg, 2-3 times a week | Inject over 1-2 minutes. |
| Ongoing | 50-100 mg weekly | Take breaks every few months. |
This is a reference schedule, not a prescription. Start at the lowest step.
Mixing it
A common setup for NAD+ is a 500 mg vial with 5 mL of bacteriostatic water.
That makes 100 mg per mL. A 50 mg dose is about 50 units on a standard insulin syringe.
Bacteriostatic water is all you need here. Acetic acid is only for powders that will not dissolve. See the mixing guide.
Open this in the calculatorWhat to watch for
- Flushing, chest tightness, or nausea if given too fast
- Injection site stinging
Stop and speak to a clinician if anything feels wrong. See the safety page.
Common questions
Why do I feel chest tightness?
You pushed too fast. Slow the injection down and it usually stops.
Can I do it in one quick shot?
No. Speed is the main cause of side effects with NAD+.
Why is the vial so large?
NAD+ is dosed in milligrams, not micrograms. It is not a typical peptide.